学术
中草药肉苁蓉和黄芪对猪早期胚胎体外发育的影响
李玉芳[1] 徐芹伟[2] 张丽[2] 张文昌[2] 冯书堂[3]
[1]黄淮学院,河南驻马店463000 [2]福建农林大学,福建福州350002 [3]北京畜牧兽医研究所,北京100094
摘 要:
利用猪体外受精的胚胎进行研究,比较了在培养基NCSU-23中添加中药成分肉苁蓉和黄芪对早期胚胎发育的影响,实验证明：（1）培养液中添加体积比0.10%的肉苁蓉和0.10%的黄芪,肉苁蓉添加组囊胚率（15.76%）显著高于空白组（9.26%）（P〈0.05）,黄芪添加组囊胚率（10.09%）与空白组（9.26%）差异不显著（P〉0.05）,表明肉苁蓉能够显著促进早期胚胎发育。（2）肉苁蓉添加浓度（V/V）为0.05%和0.10%时,囊胚率（17.02%和16.75%）显著高于空白组（0）（P〈0.01）和0.15%组（8.94%）（P〈0.05）。（3）培养液在2细胞期添加0.10%中药肉苁蓉,囊胚发育率（13.81%）显著高于4细胞期（7.53%）（P〈0.05）,比卵裂之前添加肉苁蓉（13.67%）稍高,但差异不明显（P〉0.05）。[著者文摘]
文章出处:
《江西农业大学学报》-2007年29卷5期 -805-808页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1000-2286（2007）05-0805-04
Effect of Chinese Herbal Medicine-cistanche and Radix Astragali on the Development of Early IVF Embryo
LI Yu-fang,XU Qin-wei,ZHANG Li,ZHANG Wen-chang,FENG Shu-tang （1.Huanghuai University, Zhumadian 463000, China; 2.Fujian Agriculture and Forest University, Fuzhou 350002, China; 3. Institute of Animal Seienee,Beijing 100094, China）
Abstract:
The effect of Cistanche and Radix Astragali on the development of early IVF embryo was studied, the result suggested that, （1）The blatocysterate （15.76%） of the embryos cultured in the medium containing 0.10%（V/V） Cistanche and 0.10%（V/V）Radix Astragali was higer than that of the embryos cultured in the medium without Traditional Chinese Medicine markedly（9.26%）（P〉0.05）, and it was also a little higher than that of the embryos cultured in the medium containing Radix Astragali （10.09%）（P〉0.05）.（2）The blatocyst rates of the embryos culture d in the medium containing 0.05% and the medium containing 0.10%（V/V）Cistanche （17.02% ＆ 16.75%） were higher notablely than those of the embryos cultured in the medium containing 0.15%（V/V）Cistanche （0%）（P〈0.01） or the medium containing no Cistanche（8.94%）（P〈0.05）.（3）The blatocyst rate of the embryos transferred into the medium containing 0.01%（V/V）Cistanche at 2-cell time （13.81%） was significantly higher than that at 4-cell time （7.53%）（P〈0.05） and it was also a litter higher than that before cleavage （13.67%） （P〉0.05）.[著者文摘]
Key words:
oocyte; IVF; Cistanche; early embryo
基金资助:
“十五”中国实验用小型猪资源开发与研究应用项目（2004BA717B）和基础性工作、社会公益研究专项（2003DEB6J078）
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