学术
传染性喉气管炎病毒河南株TK基因的克隆与序列分析
陈红英[1,2] 崔保安[1,2] 李新生[1] 张书松[1] 魏战勇[1,2] 崔沛[3] 金钺[1]
[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省动物性食品安全重点实验室,河南郑州450002 [3]河南省兽医防治站,河南郑州450008
摘 要:
根据传染性喉气管炎病毒TK基因的核苷酸序列设计并合成一对引物,利用该对引物PCR扩增出鸡传染性喉气管炎病毒河南株ILTV-CGI、LTV-XY及以色列疫苗株TK全长片段,并进行克隆、测序。结果显示3株ILTV的TK基因全长均为1 183 bp,包含一个开放性阅读框（1 092 bp）,编码363个氨基酸的多肽;3株ILTV TK基因核苷酸序列完全一致,并与GenBank收录的ILTV美国632强毒株、北京E2株TK基因完全一致,而与山东烟台株、英国Thorne强毒株和英国216株TK基因核苷酸同源性均为99.5%,与其他疱疹病毒TK基因核苷酸同源性在19.1%～27.2%之间。分子进化分析揭示了各毒株间的亲缘关系。[著者文摘]
文章出处:
《江西农业大学学报》-2007年29卷5期 -813-817页
栏目信息:
文献标识码:
A
文章编号:
1000-2286（2007）05-0813-05
Cloning and Sequence Analysis of TK Gene of Chicken Infectious Laryngotracheitis Virus Henan Isolates
CHEN Hong-ying,CUI Bao-an,LI Xin-sheng,ZHANG Shu-song,WEI Zhan-yong,CUI Pei, JIN Yue （1.College of Animal Husbandry and Veterinary,Henan Agricultural University, Zhengzhou 450002, China;2.Animal Food Safety Key Laboratory, Henan Province,Zhengzhou 450002, China;3.Henan Province Veterinary Preventive and Treatment Station,Zhengzhou 450008, China）
Abstract:
One pair of primers was designed and synthesized according to the TK gene nucleotide sequence（S83714）of chicken infectious laryngotracheitis virus（ILTV）.TK genes of ILTV-XY and ILTV- CG and ILTV vaccine were amplified by PCR using the primers. The purified PCR products were cloned into pGEM-T Easy vector and then sequenced. The results indicated that all of TK gene nucleotide sequences of three ILTV strains are 1 183 bp in length, which includes one open-reading frame encoding 363 amino acid residues. TK gene nucleotide sequences of three ILTV strains were identical. After comparison with other ILTV strains in GenBank datebase, the sequences were identical to the nucleotide sequence of US field isolate 632,Beijing E2 strain, shared 99.5% homology at nucleotide level with Thome strain,216 strain and Shandong yanti strain. Evolution analysis indicated evolutionary relationship among different strains of ILTV.[著者文摘]
Key words:
infectious laryngotracheitis virus; TK gene; cloning; sequence analysis
基金资助:
国家“十五”食品安全重大攻关专项（2001BA804A30-11）
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