学术
铜在铜蛋白azurin分子构象及其促口腔肿瘤细胞凋亡中的作用
王云虹[2] 柏小丽[2] 钟金[1] 王正平[2] 张洪杰[1] 高锦[1]
[1]中国科学院生物物理研究所分子生物学中心,北京100101 [2]哈尔滨工程大学化工学院,哈尔滨150001
摘 要:
为了解铜对铜蓝蛋白azurin的构象和细胞毒性的影响，用重组表达的azurin作为研究材料，对结合铜的holo—azurin和除去铜的ado—azurin采用紫外、荧光和圆二色光谱进行了对比研究，其特征性变化为：去除铜离子后，该蛋白的紫外吸收光谱在628nm的吸收峰消失，圆二色光谱出现轻微改变。采用两株p53呈阳性表达的中国人口腔癌细胞株Acc2和Acc3为研究对象，经四唑盐（MTT）比色法和流式细胞仪分析发现，azurin具有抑制上述肿瘤细胞株的生长，并促进肿瘤细胞凋亡的作用，而脱铜后的apo—azurin抗肿瘤活性降低。[著者文摘]
文章出处:
《高技术通讯》-2007年17卷2期 -185-190页
栏目信息:
分 类 号:
The importance of copper in azurin molecular conformation and its apoptotic effect on oral cancer cell lines
Wang Yunhong , Bai Xiaoli , Zhong Jin, Wang Zhengping , Zhang Hongjie , Gao Jin （ Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 ; Chemical Engineering College of Harbin Engineering University, Harbin 150001 ）
Abstract:
In order to determine the role of copper in the conformation of azurin molecule and its cvtotoxicity properties, the wild type azurin was expressed in prokaryotie system, and the spectral properties of holo-azurin compound with copper and apo-azurin devoid of copper were compared . UV spectrum revealed that absorption band of apo-azurin at 628 nm disappeared , while the fluorescence spectrum of holo-azurin and apo-azunn had no difference and only minor changes of circular dichroism spectrum for ape-azurin were observed. Biological activities of azurin on p53 positive Acc2 and Acc3 Chinese oral cancer cell lines were also investigated with MTT assay and flow cytometer. The results indicated that azurin could inhibit the cancer cell proliferation and enhance cell＇s apoptosis. Although the copper atom was not necessary, for azurin to perform its cytotoxicity and apoptosis activity in the cancer cell lines, the pharmaeological activity of ape-azurin on apoptosis was found to decrease compared to the holo-azurin.[著者文摘]
Key words:
copper, azurin, apo-azurin, ultraviolet spectrum, fluorescence spectrum, circular diehroism spectrum, aoootosis
基金资助:
中国科学院生物科学与物技术研究特别支持（财政部专项）和863计划（20033AA624010）资助项目.
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