学术
摘 要:
细菌E1R-J是一株对小麦全蚀病、小麦白粉病和小麦条锈病有拮抗作用的小麦内生芽孢杆菌，笔者对其产菌的发酵培养条件进行了初步研究。发酵培养基为酵母粉0．6％，蛋白胨0．6％，NaCl0．3％，K2HPO40．02％。最佳发酵条件为pH4．5～9．0，菌株都可以生长，转速150r／min，温度34℃，种子液的接种量为2％，通气量是空气与培养基的比例2：5，最佳种子液菌龄是20～34h，作为生防用菌最佳菌龄为48～73h。在以上条件下，细菌的发酵产量最大，细菌浓度为5．2×10^7cfu／mL左右。[著者文摘]
文章出处:
《西北农业学报》-2008年17卷1期 -61-64页
分 类 号:
文献标识码:
A
文章编号:
1004-1389（2008）01-0061-04
Optimizing of Fermentation Conditions of Endophytic Bacteria E1R-J
GONG Yu-fei, QIAO Hong-ping, WEI Guo-rong, GAO Xiao-ning, HUANG Li-li , KANG Zhen-sheng （College of Plant Protection, Northwest A＆F University, Yangling Shaanxi 712100, China）
Abstract:
The endophytic bacteria EIR-J is isolated from the root of winter wheat, which has the better effect to take-all, powdery mildew and rust of wheat. And that the optimizing of the fermentation conditions showed that the optimum media is that made of peptone 0.6%, yeast exact 0.6%, NaCl 0.3%, K2HPO4 0.02% with initial pH 4.5-9.0, culturing at 34℃, 150 r/min, and inoculation of seeds culture with 2℃, in 100 mL LB of 250 mL flask, the time of seeds culture is 20-34 h, and the time of fermentation for biocontrol is 48-73 h. So the concentration is 5.2 ×10^7cfu/mLo[著者文摘]
Key words:
Bacillus subtilis ; Optimizing ; Fermentation conditions ; Optimum media
基金资助:
教育部“长江学者与创新团队”发展计划项目（200558）;国家科技支撑计划（2006BAD08A05）;高等学校学科创新引智计划资助项目（B07049）;国家973项目（200611D100203）;国家“十五”攻关项目（2001BA509B03）.
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