学术
大口径毛细管气相色谱分离和测定银杏叶萜内酯的方法建立
廖咏玲[1] 许锋[1] 朱俊[1] 王燕[1] 程水源[1,2]
[1]长江大学园艺园林学院,湖北荆州434025 [2]黄冈师范学院生命科学与工程学院,湖北黄冈438000
摘 要:
为建立灵敏、简便测定银杏叶萜内酯气相色谱法，试验采用大口径毛细管气相色谱法，对银杏叶中的银杏内酯A（GA）、银杏内酯B（GB）、银杏内酯C（GC）和白果内酯（BB）4种银杏萜内酯进行了分离与测定。用20％的甲醇水溶液提取银杏叶中的银杏内酯A，B，C和白果内酯，乙酸乙酯（EOA）／四氢呋喃（THF）一步纯化，衍生后进行GC-FID检测。样品在16min内完全分离，GA、GB、GC和BB的相对标准偏差均小于0．8％，平均回收率为94．45％～98．98％。本方法测定银杏叶萜内酯简便、灵敏、准确、重现性好。[著者文摘]
文章出处:
《西北农业学报》-2008年17卷1期 -146-149页
分 类 号:
文献标识码:
A
文章编号:
1004-1389（2008）01-0146-04
Separation and Determination of Terpene Trilactones by Gas Chromatography with Wide Bore Capillary Column
LIAO Yong-ling , XU Feng, ZHU Jun, WANG Yan,CHENG Shui-yuan （1. College of Horticulture and Gardening, Yangtze University, Jingzhou Hubei 434025, China;2. College of Life Science and Engineering, Huanggang Normal University, Huanggang Hubei 438000, China）
Abstract:
In order to determined terpene trilactones simplely and rapidly from Ginkgo biloba leaves. A method for the simultaneous separation and determination of terpene trilactones from Ginkgo biloba leaf by gas chromatography with wide-bore capillary column was described. The results showed that the components were extracted from Ginkgo biloba leaves with 20% methanol in water, the extracts were dissolved in 10% NaH2PO4 solution under ultrasonication and separated from the solution by extraction with a mixture of ethyl acetate/tetrahdrofuran in a capped vial. After derivatization, these terpene trilactones could be quantified and qualitative analyzed using GC-FID. All components were separated in 16min. The relative standard deviations were less than 0.8%, and the average recoveries were 94.45%-98.98%. The method is simple, rapid, sensitive and reproducible.[著者文摘]
Key words:
Wide bore capillary column; Gas chromatography; Ginkgo terpene trilaetone; Extraction Detection
基金资助:
湖北省重大科技攻关项目（2004AA204B03）;荆州市科技发展项目（20041PB06）;湖北省自然科学基金（2006ABA005）.
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