学术
摘 要:
微卫星是以几个碱基为重复单位组成的简单串联重复序列，具有丰度高、多态性高、共显性标记、选择中性、可自动检测等优点，作为第二代分子遗传标记，已经在很多领域得到广泛应用。微卫星位点可以提供具高分辨率的遗传信息，这一特点使微卫星适合于个体水平上的研究，可进行个体之间的亲缘关系分析包括亲子鉴定等。本文着重介绍了微卫星在体细胞克隆动物研究中的应用。[著者文摘]
文章出处:
《家畜生态学报》-2006年27卷4期 -14-17页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1004-5228（2006）04-0014-04
Application of Microsatellites in the Study of Somatic Cell-Cloned Animals
WANG Zheng-chao, PANG Xun-sheng, PAN Xiao-yan, WANG Feng, SHI Fang-xiong （Department of Animal Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China）
Abstract:
Microsatellites are simple tandem of repeated sequence motifs consisting of repeated units of several base pair in length. As genetic markers, they are widely dispersed in eukaryotic genomes. The advantages of microsatellites include high polymorphism and abundance, co-dominance, selective neutrality and the possibility of automated detection and scoring. As one of the second generation of molecular genetic markers, they have been used in a wide range of applications which were previously difficult to address experimentally. Microsatellite loci is so variable that most individuals have unique multilocus genetypes, even with a small number of loci and a large number of individuals. Therefore, it is possible to study parentage test at individual levels and to perform the parentage relation analysis. This article emphasizes the applications of microsatellites in the studies of somatic cell-cloned animals.[著者文摘]
Key words:
Microsatellites ; Cloned Animal; Genetic marker
基金资助:
安徽省自然科学基金（03041203）资助.
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