学术
波尔山羊FSHR 5’端序列的SNP多态性及超排效果分析
宋美玲[1] 马向明[2] 王建民[3]
[1]中国农业大学动物科技学院,北京100094 [2]山东省东营市科技局,山东东营257345 [3]山东农业大学动物科技学院,山东泰安271018
摘 要:
为探索波尔山羊超排个体排卵数差异与个体遗传特性的相关性，对波尔山羊的FSHR基因（950bp），分成四段分别设计引物进行PCR-SSCP多态性分析，结果发现一个SNP位点，导致5’-1出现两种基因型。序列分析显示该突变位，最位于转录起始点上游-739bp处，属于远端转录启动调控区。统计分析表明AA型个体的平均排卵数与AB型个体的平均排卵数差异极显著（P〈0．01），平均胚胎可利用率差异显著（P〈0．05），这说明个体的超排潜力可能与其本身的FSHR基因的遗传特性有关。[著者文摘]
文章出处:
《家畜生态学报》-2006年27卷4期 -25-28页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1004-5228（2006）04-0025-04
Single Nucleotide Polymorphism in Goat FSHR Gene and Its Association with Superovulation
SONG Mei-ling, MA Xiang-ming, WANG Jian-min（1. College of Animal Science and Technology ,China Agriculture University, Beijing 100094; 2. Bureau of Science and Technology,Dongying 257345; 3. College of Animal Science and Technology ,Shandong Agriculture University, Taian 271018）
Abstract:
The Boer goat FSHR gene was analyzed with single nucleotide polymorphism（SNP） by four primers. The results showed that there was a SNP locus at the 5 ＇-1 which the mutation taken placed in - 739bp upstream from the transcriptional location G to A, so there were two genotypes： AA and AB, and the average number of multiplying ovulation was obviously different between the different genotypic individuals.[著者文摘]
Key words:
boer goat; FSHR receptor; 5＇ flanking regulating; single nucleotide polymorphism; superovulation
基金资助:
山东省农业良种开发肉羊项目（SD20030708）资助.
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