学术
摘 要:
用不同酶消化分离山羊小肠上皮细胞，从而找到最佳的分离方法，为进一步研究IEC提供合适的细胞模型。本研究分别用胶原酶工、嗜热菌蛋白酶、胶原酶Ⅺ／中性蛋白酶I消化分离刚出生未进奶的山羊小肠上皮细胞，通过观察IEC生长状态、形态特征、抗原鉴定比较哪种分离效果最佳。结果表明，联合用胶原酶Ⅺ／中性蛋白酶I1h可分离出大量健全绒毛隐窝单位，进一步培养获得的小肠粘膜上皮（IEC），上皮细胞膜抗原均呈阳性，用胶原酶工和嗜热菌蛋白酶分离得到的大部分是成纤维细胞。选用用胶原酶Ⅺ／中性蛋白酶工作为分离IEC的消化酶，可获得较纯的IEC。[著者文摘]
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文章出处:
《家畜生态学报》-2007年28卷6期 -9-12页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1004-5228（2007）06-0009-04
Comparison of Methods to Generate Vialde Goat Intestinal Epithelial Call by Enzymes
WANG Hai-xia ,KUANG Wei, ZHAO Guo-qi（College of Animal Science and Technology, Yangzhou University ,Yangzhou 225009, China）
Abstract:
To find the optimal method to isolate and culture the goat intestine epithelial cells,collagenase I . thermolysin.collagenaseXI/dispase I digestion technique were used to isolate IEC from the fetal goat. Optimal culture conditions were determined by both cellular morphology under light microscope and 4 immunocytochemical characterization. The results showed that large amount of villi crypt units were attainable and the attached cells were positive for cytokeratin using collagenaseXI/dispase I digestion technique while many fibroblast cells contaminated when using collagenase I and thermolysin digestion techniques. This study showed that viable epithelial cells can be relatively easily generated from fetal goat small intestine by collagenaseXI/dispase I digestion technique.[著者文摘]
Key words:
goat intestine epithelial cells; collagenase I; collagenaseⅪ/dispase I ; thermolysin
基金资助:
国家自然基金（编号：30471259）.
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