学术
牙鲆5个养殖群体的遗传多样性分析
刘海金[1,3] 朱晓琛[2] 孙效文[3] 杨立更[2] 薛玲玲[1,3] 毛连菊[4]
[1]中国水产科学研究院,北京100039 [2]中国水产科学研究院北戴河中心实验站,河北秦皇岛066100 [3]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [4]大连水产学院生命科学与技术学院,辽宁大连116023
摘 要:
利用16对微卫星分子标记对牙鲆（Paralichthys olivaceus）的5个养殖群体进行遗传多样性分析。结果表明，等位基因数为2-9个，平均等位基因数为6．0625；有效等位基因数1．2596-5．5161，平均有效等位基因数3．692；各位点的杂合度观测值（Ho）0．2200-0．8000；杂合度期望值（He）0．2061-0．8187。各群体之间无偏倚杂合度期望值从小到大依次为丹东、北戴河、威海、青岛、荣成。Kruskal-Wallis检验结果（H=0．672，df=4，P=0．955）则说明，5个群体遗传多样性差异不显著。群体间基因分化系数（Gs，）为0．0991，各群体之间存在中度遗传分化。采用UPGMA法对5个群体进行聚类，可分3类：丹东和北戴河各为一类，威海、荣成、青岛为一类，其中威海与荣成群体的分化最小。结合Hardy-Weinberg平衡，拟合度检验和遗传偏离指数（动的分析结果表明，各群体的遗传平衡状况存在很大差别。遗传变异的分析结果说明5群体遗传多样性比较丰富。[著者文摘]
文章出处:
《中国水产科学》-2008年1期 -30-37页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1005-8737-（2008）01-0030-08
Analysis of genetic structure among 5 cultured stocks of Japanese flounder Paralichthys olivaceus
LIU Hai-jin, ZHU Xiao-chen, SUN Xiao-wen, XUE Ling-ling, ZHAO Ying-ying , MAO Lian-ju （1. Chinese Academy of Fishery Sciences, Beijing 100039, China; 2. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China; 3. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 4. College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China; 5. Life Science and Technology Institute, Dalian Fisheries University, Dalian 116023, China）
Abstract:
Genetic divergence within and between 5 cultured stocks （Dandong, Beidaihe, Weihai, Rongcheng and Qingdao） of Japanese flounder （Paralichthys olivaceus） was assessed by 16 microsatellite loci which in the 5 cultured populations showed allele number （A） was 2 to 9; average allele number was 6.062 5; effective allele number （Nc） was 1.259 6-5.516 1 ： averge effective allele number was 3.692; observed heterozygosity value （Ho） is 0.2200 to 0.8000： excepted heterozygosity value （He） was 0.206 1 to 0.818 7. Hevalues followed the order of Dandong, Beidaihe, Weihai, Qingdao, Rongcheng, and a comparison by Kruskal-Wallis test revealed that there was no significant difference in genetic diversity of the 5 cultured stocks （H=0.672, df=4, P=0.955）. The results also showed that the coefficient of gene differentiation between the stocks （GST） was 0.0991, there were moderate genetic differentiation among these 5 cultured populations. The genetic distance of 5 cultured populations was calculated and the cluster analysis by UPGMA method was carried out that all populations were pooled into three groups： Dandong, Beidaihe and Shandong （including Weihai, Rongcheng and Qingdao）. Among the 3 populations of Shandong, Weihai and Rongcheng were more similar. Hardy-Weinberg equilibrium analysis in 16 microsatellite loci by Chi Square test and genetic deviation index （d） showed a remarkable difference in populations. [Journal of Fishery Sciences of China, 2008, 15 （1） ： 30-37][著者文摘]
Key words:
Japanese flounder; cultured population; genetic diversity; microsatellite
基金资助:
国家科技攻关项目（96-920-44-01）;国家科技支撑计划（2006BAD01A1207）;农业科技跨越计划（2006跨18）
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