学术
摘 要:
研究操作胁迫（追赶惊扰）对大黄鱼（Pseudosciaena crocea Richardson）幼鱼的生长、行为、肝脏、脾脏、免疫功能等方面的影响。实验用大黄鱼幼鱼全长（5．85±0．45）cm，实验共设3个组：对照组、实验组1（每日操作胁迫1次）、实验组2（每日操作胁迫2次），每组2个重复。分别于实验开始后第10天、20天、30天，取样测定其全长、体质量、脾脏重以及肝脏重，计算脾系数和肝系数；断尾取血做血涂片，在油镜下进行白细胞分类计数，并对各项指标进行统计分析。结果表明，长期的剧烈操作胁迫会显著抑制大黄鱼幼鱼的生长，同时也影响其肝脾脏功能和机体免疫机能，如增加大黄鱼幼鱼脾系数（即脾肿大），肝脏的合成代谢先受到抑制；各种白细胞比率受剧烈操作胁迫（每日2次胁迫）影响发生显著变化，嗜中性粒细胞和单核细胞在实验前期（10d）或中期（20d）显著增多，而淋巴细胞则明显减少，但在实验末期均又恢复正常水平，其原因可能是大黄鱼的免疫机能对长期操作胁迫表现一定的适应性。[著者文摘]
文章出处:
《中国水产科学》-2008年1期 -100-105页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1005-8737-（2008）01-0100-06
Effect of handling stress on fingerlings of large yellow croaker （Pseudosciaena crocea Richardson）
WENG Zhao-hong, WANG Zhi-yong, XIE Fang-jing, XIE Yang-jie （Fisheries College, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Institute of Aquaculture Biotechnology, Jimei University, Xiamen 361021, China）
Abstract:
The effects of acute handling stress （ chasing and startling） on fingerlings of large yellow croaker （Pseudosciaena crocea Richardson） were studied by the response of growth rate, behavior, immunity, spleen and liver functions offish. Totally three experimental groups, including treatment 1 （stressed one time each day）, treatment 2 （stressed twice daily） and control group, were set and the fish were sampled at day 10, day 20 and day 30 after handling stress. The levels of response to acute handling stress were investigated from the following aspects： body length, body weight, weights of spleen and liver, the ratio of spleen weight to body weight and the hepatosomatic index. Blood smears were also made to calculate the number and percentages of different kinds of leucocytes. The results showed that the frequently acute handling stress not only significantly reduced growth rate of large yellow croaker fingerlings, but also affected the functions of spleen and liver. The phenomena of spleenswelling and restraining liver anabolism were investegated in the treated fish. During the pre- and mid- stage （dayl0 and day 20） of experiments in treatment 2, the percentages of neulrophils and monocytes increased markedly, but those of lymphocytes decreased significantly, and finally all of them got back to the normal levels at the last experiment stage. The data suggested that the fish could adapt to handling stress to some extent for its immunol founction. [Journal of Fishery Sciences of China, 2008, 15 （1）： 100-105][著者文摘]
Key words:
Pseudosciaena crocea Richardson; handling stress; stress
基金资助:
国家863计划项目（2002AA603201）;福建省重大科技项目（20012009）;福建省教育厅项目（JA01085）.
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