学术
摘 要:
研究了一种用蒸馏法提取，高效液相色谱法测定食醋中苯甲酸和山梨酸的方法。用蒸馏法提取食醋中的苯甲酸和山梨酸。收集蒸馏液直接进行液相色谱分析。使用的色谱柱为C柱，流动相为甲醇-乙腈-5mmol／L柠檬酸缓冲溶液（体积比为10：20：70），检测波长为230nm。苯甲酸和山梨酸标准溶液在0．25mg／L-100mg／L内线性良好，其线性相关系数分别为0．9999和0．9998，加标回收率为91．0％-95．2％，测量结果的相对标准偏差为2．46％～3．45％。该方法用于测定食醋样品中的苯甲酸、山梨酸，检测下限均为0．5Ing／L。[著者文摘]
文章出处:
《食品工程》-2007年4期 -53-54页
栏目信息:
分 类 号:
Determination of benzoic acid and sorbic acid in vinegar by high performance liquid chromatography
Zhang Wei-jia Jia Qi-bin Xia Tian-lan （College of bioteehnology ,XiHua university, Chengdu 610039,China）
Abstract:
Benzoic acid and sorbic acid in vinegar were extracted by distillating and determined by high perform ance liquid chromatography （HPLC）.A C18 column was used with the mobile phase of naethanol-acetonitrile-5 nanaol/L citric acid solution （10：20 ： 70）and the detection wavelength of 230 nna. The calibration curves presented good linear relationship in the range of 0.25nag / L ~ 100 nag / L, and the correlation coefficients were 0.9999 and 0.9998 for benzoic acid and sorbic acid respectively. Recoveries of benzoic acid and sorbic acid added were 91.0 % - 95.2 % .Detection limit of the method for benzoic acid and sorbic acid was 0.5 mg / L.[著者文摘]
Key words:
High performance liquid chromatogTaphyVinegar Benzoic acid Sorbic acid
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