学术
摘 要:
本文旨在探讨牛膝多糖（ABPS）对断奶仔猪抗菌肽Protegrin-1（PG-1）mRNA表达的影响。试验采用单因子设计，按照日粮处理因素不同，设对照组、抗生素组、0．05％、0．10％和0．15％牛膝多糖组，共5个处理。选用28日龄社长大仔猪160头，随机分人5个处理，每个处理4个重复，每个重复8头猪（公母各半）。采用半定量RT-PCR法，以18SrRNA为内标，检测PG-1mRNA相对表达量。结果表明：日粮中添加牛膝多糖均不同程度地促进断奶仔猪骨髓抗菌肽PG-1mRNA的表达。与对照组相比，0．05％、0．10％和0．15％牛膝多糖组分别使断奶仔猪PG-1mRNA的相对表达量增加14．9％（P〉0．05）、36．4％（P〈0．05）和7．3％（P〈0．05）；与抗生素组相比，其PG-1mRNA的相对表达量分别增加7．8％（P〉0．05）、27．9％（P〉0．05）和19．4％（P〉0．05）。即牛膝多糖能显著促进断奶仔猪骨髓抗菌肽PG．1mRNA表达，且日粮中以0．10％添加量为适宜。[著者文摘]
文章出处:
《动物营养学报》-2008年20卷1期 -80-84页
栏目信息:
The Effect of Achyranthes Bidentata Expression in Polysaccharides on Protegrin-lmRNA Weaned Piglets
ZHA0 Yu-rong ,CHEN Qing-hua, HE Jian-hua, FAN Zhi-yong, ZH0U Can, LIU Zhu-ying （ College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China）
Abstract:
To investigate the effect of A.chyranthes bidentata polysaccharides（ABPS） on expression of antibacterial peptide Protegrin-1 （PG-1） mRNA in weaned piglets,according to the different dietary factors, five test groups were used, including control group, antibiotics group, 0,05%, 0.10% and 0, 15% ABPS groups. One hundred and sixty weaned piglets （Duroc x （Landrace x Yorkshine）） with age of 28 days were randomly allotted to five test groups with four replicates in each group and eight weaned piglets in each repeat. An optimized semi-quantitative RT-PCR was constructed to detect the relative ex- pression levels of PG-1 mRNA by using 18S rRNA as inner control. The results showed that PG-1 mRNA expression could bc enhanced to a different extent with ABPS in weaned piglets, Comparcd with control group, 0.05%, 0.10% and 0, 15% AB- PS enhanced the PG-1 mRNA expression by 14.9 % （ P〉0.05）, 36.4% （P〈0.05） and 27.3 % （ P〈0.05） respectively; Compared with antibiotics group, 0. 05%, 0. 10% and 0. 15% ABPS increased the PG-1 mRNA expression by 7.8% （P〉 0.05 ）, 27.9% （P〉0.05） and 19.4% （P〉0.05） respectively. Above results suggested that, ABPS remarkably increased rel- ative expression of PG-1 mRNA in femur marrow of wcaned piglets, and the appropriate ABPS addition was 0.10%.[著者文摘]
Key words:
Semi-quantitative RT-PCR ; Achyranthes bidentata polysaccharides ; Weaned piglets ;Antibacterial peptide ; Prote-grin- 1 ; Gene expression
基金资助:
湖南省重大专项（05NK003）;湖南省自然科学基金项目（06JJ50070）;湖南农业大学人才引进基金项目（YJ0508）
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