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Methods in EnzymologyEscherichia ColiProteinsProtein InteractionsEnzymeSaccharomyces CerevisiaeLiving CellsGreen Fluorescent Proteinin VivoMammalian CellsMembrane ProteinsYeastin Vitro and in VivoNUCLEAR MAGNETIC RESONANCEExpression and PurificationAffinity ChromatographyMass SpectrometryAssay MethodSecondary StructureSpecific Activity