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Synthetic BiologyMetabolic EngineeringDirected EvolutionSaccharomyces CerevisiaeEscherichia ColiProtein EngineeringGenetic CircuitsGenetic CircuitGenome EngineeringGene ExpressionGENOME EDITINGGene CircuitsProtein SynthesisHeterologous ExpressionCell-free Synthetic BiologyDNA AssemblyTranscription and TranslationEngineered Escherichia ColiControl of Gene ExpressionCell-free Expression